Abstract

FREE RADICAL SCAVENGING ACTIVITY OF 4-HYDROXYPROPIOPHENONE BY IN VITRO ASSAYS

P. R. Kiresee Saghana and S. Hemalatha*

ABSTRACT

The present study concentrated on deciding the free radical scavenging activity of 4-hydroxypropiophenone (4-HPPP) through in vitro free radical scavenging tests. 4-HPPP was determined by using, 1,1-diphenyl-2-picryl-hydrazyl(DPPH), 2’-Azinobis-(3-Ethylbenzothia zoline-6-Sulfonic Acid) (ABTS+), Ferric reducing antioxidant power (FRAP), nitric oxide scavenging assay (NO), reducing power, superoxide anion radical scavenging (SOD), hydroxyl radical scavenging assay (HRSA), metal chelating activity, phosphomolybdeneum and hydrogen peroxide assay. A score of bioavailability and medication resemblance properties of 4-HPPP has a decent score exclusively. Free radical scavenging potentiality of DPPH (37.74μg/ml) compared to standard drug Ascorbic acid (AA) (35.62μg/ml) respectively, ABTS (62.31μg μg/ml) and AA (57.74μg/ml), FRAP (42.79μg/ml) and AA(39.74μg/ml.), NO (45.28μg/ml) and AA (49.74μg/ml), reducing power (39.09μg/ml) and AA (34.74μg/ml), hydroxyl (36.57μg/ml) and (31.74μg/ml), superoxide (54.48μg/ml) and AA (50.74μg/ml), and hydrogen peroxide (68.74μg/ml) and AA(74.02μg/ml), metal chelating(37.90μg/ml) and AA (55.74μg/ml) and also phosphomolybdneum (43.35μg/ml) and AA (38.74μg/ml) respectively, for the 4-HPPP. The present investigation clearly indicated that the 4-HPPP possesses antioxidant properties and serve as free radical inhibitors or scavengers, acting possibly as primary antioxidants.

Keywords: Free radical scavenging, 4-HPPP, DPPH and superoxide scavenging.