Abstract

DETECTION OF ETHYL PARATHION, METHYL PARATHION AND FENITROTHION IN BIOLOGICAL MATERIAL BY THIN LAYER CHROMATOGRAPHY

Sanjay. S. Prabhavale* and B. B. Daundkar

ABSTRACT

In this paper a new chromogenic spray reagent has been developed for Ethyl Parathion, Methyl Parathion and Fenitrothion by thin layer chromatography. These insecticides on reduction with stannous chloride in 50 % hydrochloric acid give respective amino derivatives. These amino derivatives further react with 1, 2 napthoquinone-4-sulfonic acid yield red-orange coloured para-quinoidal condensation product. Other organophosphorous, organochloro, carbamate and synthetic pyrithriod insecticides do not reacts with this reagents. Hence it is a simple and selective chromogenic reagent for organophosphorous insecticides containing nitrophenyl group.

Keywords: TLC, Biological tissue, Ethyl Parathion, Methyl Parathion, Fenitrothion, Stannous chloride, 1, 2 napthoquinone - 4-sulfonic acid.