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Abstract

METHOD DEVELOPMENT AND VALIDATION OF CEFPODOXIME PROXETIL IN IMMEDIATE RELEASE DOSAGE FORM BY USING RP-HPLC

N.Gopi*, D.Narasimha Rao1, M.Prasad Rao1, Sivashankar.R.Beeravalli2

1Department of Pharmaceutical Analysis, M.A.M College of Pharmacy, Kesanupalli, Narasaraopet,Guntur-522601
2inkwood Drive, Fredericks Burg, Virginia-22407.

ABSTRACT

A simple, sensitive and rapid reverse phase high performance liquidchromatographic method was developed for the estimation ofCefpodoxime proxetil in pure and in pharmaceutical dosage form. AHypersil BDS C18 (250 x 100 mm, 4.5m) was used with a mobilephase containing a mixture of and Potassium dihydrogen phosphateand Acetonitrile buffer adjusted to Ph6.5+ 0.1 with PotassiumHydroxide in the ratio of 62:38. The flow rate was 2.0ml/min andeffluent was monitored at 235nm and peaks eluted at 7.7min, 8.7min.Calibration curve was plotted with a range from 17.5-87.51 μg/ml. Theassay was validated for the parameters like linearity, precision,accuracy, robustness and system suitability parameters. The proposedmethod can be useful in the routine analysis for the estimation ofCefpodoxime Proxetil in pharmaceutical dosage form.

Keywords: Cefpodoxime Proxetil, RP-HPLC.


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