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Abstract

CELLULASE PRODUCTION BY TRICHODERMA HARZIANUM AND FUSARIUM OXYSPORUM UNDER SOLID STATE FERMENTATION

Mangalanayaki R.* and Madhavan S.

ABSTRACT

The enzyme activities of Trichoderma harzianum and Fusarium oxysporum grown on various waste cellulosic materials such as Cotton seed, corn cob and rice husk were tested for a period of 192 hrs. The maximum activity of 0.40 mg/min/ml & 0.030 mg/min/ml of cellulase enzyme were found to be produced on cotton seed after 144hrs by Trichoderma harzianum and Fusarium oxysporum respectively. Corn cob showed the highest activity at 120 hrs of 0.32 mg/min/ml & at 96 hrs of 0.0232 mg/min/ml by Trichoderma harzianum and Fusarium oxysporum, followed by rice husk with the highest activity of 0.024 mg/min/ml & 0.0242 mg/min/ml 120 hrs by Trichoderma harzianum and Fusarium oxysporum respectively. From the above results, Trichoderma harzianum produced the highest amount of cellulase activity with wheat bran as substrate followed by Fusarium oxysporum by solid state fermentation.

Keywords: Fusarium oxysporum, Trichoderma harzianum, SSF and Cellulase.


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