Abstract

ANTIBACTERIAL AND PHYTOCHEMICAL ANALYSIS OF CISSUS QUADRANGULARIS ON SELECTED UTI PATHOGENS AND MOLECULAR CHARACTERIZATION FOR PHYLOGENETIC ANALYSIS OF KLEBSIELLA PNEUMONIAE

G. Vijayalakshmi, Dr. O. S. Aysha* and Dr. S. Valli

ABSTRACT

Cissus quadrangularis is a common perennial climber of family Vitaceae, particularly found in tropical regions and also distributed throughout India. It is a fleshy, cactus like liana widely used as a common food item in India. The present study was designed to evaluate the antibacterial activity of methanol, ethyl acetate and hexane stem extracts of Cissus quadrangularis against selected UTI bacterial pathogens such as Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa by in vitro agar well diffusion method. The ethyl acetate extract of the plant was found to possess strong antibacterial activity against tested pathogens. The ethyl acetate extract exhibited maximum activity against Klebsiella pneumoniae with zone of inhibition 25mm. The preliminary phytochemical analysis was performed in ethyl acetate extract. The yield was maximum compared
to methanol and hexane. The phytochemical test indicates the presence of carbohydrates, saponins, flavonoids, alkaloid, glycoside and phenols in the plant components and the active components were identified by GCMS. Klebsiella pneumoniae which was inhibited maximum has been subjected to molecular characterization for phylogentic analysis. The DNA sequencing was carried out. BLAST analysis and the phylogenetic analysis was carried by PHYLIP software programe. The sequence alignment representation and phylogenetic analysis clearly indicates the genome was Klebsiella pneumoniae

Keywords: Cissus quadrangularis, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa.