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Abstract

GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM BANANA INFLORESCENCE AND DEVELOPMENTAL TOXICITY EVALUATION OF NANO PARTICLE BY USING DANIO RERIO (ZERBRAFISH) EMBRYOS

Sowmya S.* and Geetha Viswanathan

ABSTRACT

The application of nanotechnology is one of the most vigorous researches in present science and technology. Environment affable methods of synthesize nanoparticles are demanding research in the branch of nanotechnology. The present research leads to the synthesis of nanoparticles from silver nitrate through the methanol extract of banana inflorescence as reducing, as well as capping agent. Synthesized nanoparticles were characterized under UV-vis spectroscopy at the range of 400-450nm. The peak showed at 430nm. The chemical groups studied using FT-IR, SEM analysis. Synthesized silver nanoparticle was carried out to evaluate the acute toxicity, LC50 and teratogenicity using zebrafish embryos. Only higher concentrations (700 μg to 900 μg) showed considerable mortality and delay in hatching, LC50 values of approximately 3.5 mg/L. Overall, there was no significant toxicity observed in zebrafish development when compared to control embryos. This will help us to determine the dosage and toxicity of synthesized nanoparticles for further studies. Based on the result obtained with further analysis, synthesized silver nano-particle can be utilized in various fields such as cosmetology, dermatology, nanotechnology and so on.

Keywords: Cosmetology, FT-IR, Danio rerio embryos, toxicology.


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