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Abstract

DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR ESTIMATION OF GLIBENCLAMIDE IN NANOEMULSION FORMULATION.

Mohammad Wais*, Mohd Aqil

ABSTRACT

To develop and validate a simple and rapid reversed phase high performance liquid chromatographic (RP-HPLC) method for the determination of glibenclamide (GLBD) in nanoemulsion formulation. The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC The development and validation work was carried out on a HPLC system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of system consisting of Binary HPLC pump and 2998 UV array detector. The column used was The column used was The column used was The column used was The column used was The column used was The column used was The column used was The column used was The column used was The column used was The column used was The column used was The column used was The column used was The column used was The column used was The column used was C18 (250 × 4.6 mm) 5μ ID. The HPLC system The HPLC system The HPLC system The HPLC system The HPLC system The HPLC system The HPLC system The HPLC system The HPLC system The HPLC system The HPLC system The HPLC system The HPLC system The HPLC system was eq was eqwas eqwas eqwas equilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water uilibrated with the mobile phase consisting of acetonitrile/water (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at (volume by volume) and the chromatographic peaks were detected at 228 nm. 228 nm. 228 nm. GLBD has been eluted at 6.36 min. Linearity was obtained over a concentration range of 5–50 μg/ml (r2>0.999). The Limit of detection (LOD) and Limit of quantitation (LOQ) were found to be 0.1 μg/ml and 05. μg/ml respectively. The mobile phase consisted of acetonitrile and water in the ratio of (60: 40, v/v), respectively. The analysis time was 10 min at a 1.0 ml/min flow rate. The UV detection was carried out at 228 nm. The RP - HPLC assay method developed for GLBD is rapid, precise, accurate, and specific. The method may be used for assessing the stability of GLBD as a bulk drug and in its pharmaceutical nanoemulsion formulations. The RT less than 10 min which was found to be practicably advantageous for the use of this method in routine analysis.

Keywords: RP-HPLC, GLBD nanoemulsion.


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