Abstract

EVALUATION OF ANTIDIABETIC ACTIVITY OF ALCOHOLIC EXTRACT OF ALOE BARBADENSIS, MOMARDICA CHARANTIA, TRIGONELLA FOENUM-GRAECUM AND THEIR COMBINATIONS USED IN ALLOXAN INDUCED DIABETIC RATS

G. Abhinayani*, V. Soma Shanker and Fathima Benazir

ABSTRACT

Diabetes is one of the most common non-communicable diseases and a serious life-long condition appearing worldwide. The objective of the study is to demonstrate the antidiabetic activity of the alcoholic extracts of Momardica Charantia, Aloe Barbadensis and Trigonella Foenum-Graecum and their combination were used in alloxan-induced diabetic albino rats. The powdered leaves of Momardica Charantia, Aloe Barbadensis and Trigonella Foenum-Graecum were successively extracted in 100-150ml each of alcohol by using a soxhlet apparatus. The rats after alloxination were given 5%w/v glucose solution in feeding bottles for the next 24hrs in their cages to prevent hypoglycaemia. After 72hrs, rats with fasting blood glucose levels greater than 200mg/dl were selected and used for the study. The blood samples were collected through tip of tail vein at 0, 1, 2, 4 and 8 hrs respectively after administration. The treatment was continued for next 22days. The blood glucose level was estimated at various intervals by using glucometer. The difference observed between the initial and final fasting blood glucose levels of extract treated hyperglycaemic rats revealed the antihyperglycemic effect of Momardica Charantia, Aloe Barbadensis and Trigonella Foenum-Graecum and their combination throughout the period of study. The effect of three different extracts and their combinations are compared to that of reference standards, glibenclamide was found to be significant. In conclusion, these extracts showed significant anti-diabetic effect in diabetic rats after I.P administration. The drug has the potential to act as an anti-diabetic drug in combination therapy.

Keywords: Alloxan, Anti-diabetic activity, Glibenclamide, Momardica Charantia, Aloe Barbadensis and Trigonella Foenum-Graecum.