Abstract

IN-VITRO PROTEIN BINDING STUDY OF SULPHAMETHOXAZOLE -NEW METHOD DEVELOPMENT AND ANALYSIS BY UV SPECTROPHOTOMETER

P. Ramasubramaniyan*, S.C. Rajesh, P. Solairaj, C. Shibin Raj, Nadar Anton Prisca Jeyapaul

ABSTRACT

A rapid, reliable and sensitive UV-spectrophotometric method has been developed for the determination of the Sulphamethoxazole and its in-vitro protein binding studies. The sulphamethoxazole shows λmax at 256 nm and the linearity plot yielded a correlation coefficient (R2) of 0.9997 over the Beers range of 1–10μg/ml. The developed method was employed in in-vitro protein binding studies, the drug shows 72% binding and it was good pharmacokinetic data and drug release kinetics.

Keywords: sulphamethoxazole, rapid, reliable and sensitive UV-spectrophotometric.