Abstract

IN VITRO MICROPROPAGATION AND CALLUS CULTURE OF SPILANTHES ACMELLA MURR

Sangeeta Soni*, Tulika Kangsabanik*, Shagufta Khan, U. N. Soni

ABSTRACT

Spilanthes acmella plants have been extensively used for human disease management since early ages, due to presence of valuable chemical identities which are used for the treatment of teeth ache, sexual disorder, mouth paralysis etc, but still it has remained unexplored so the present study is a new innovation for betterment of human health. A rapid protocol for in-vitro propagation of S.acmella Murr has been established, for callus induction for the production of secondary metabolite. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) α-naphthalene acetic acid (NAA), Benzyl amino purine (BAP) and Indole-3-butryic acid (IBA), 2 4-dichlorophenoxyacetic acid (2,4-D) 0.1-1.0 mg-L used singly or in combination with coconut milk (CM), polyvinyl pyrrolidone (PVP), adenine sulphate (AS) and casein hydrolysate (CH). Leaf explants of S.acmella showed moderate to good amount of callus. It was observed that the medium with 0.5 BAP mg/l showed best result for initiation of shoot & shoot multiplication responded best in combination of 0.5 BAP + 0.5 NAA and also in 0.1 BAP + 0.1 NAA. Axillary shoots developed in cultures in presence of cytokines lack roots. To obtain full plants the shoots were transferred to a rooting medium & MS medium supplemented with IBA 0.5 mg/l. The leaf segments were inoculated on MS medium supplemented with various concentrations of auxins & cytokines. However it was observed only 2,4-D (auxins) at high concentration gave best result.

Keywords: S. acmella Murr, callus induction, ?-naphthalene acetic acid (NAA), Benzyl amino purine (BAP), 2, 4-dichlorophenoxyacetic acid (2, 4-D).