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K. Rajashekar, P. J. Prasuna Sundari* and Dr. Prathima Srinivas


Woodfordia fruticosa (Family-Lythraceae) is an important traditional medicinal plant. Scientific investigations reported antimicrobial, anti-inflammatory, hepatoprotective, analgesic, wound healing and antifertility activity. Phytosome technology has proved to be beneficial in providing better absorption and bioavailability of polar biomolecules over conventional herbal extracts. The aim of the present study was to prepare and evaluate topical phytosomal gel of woodfordia fruticosa extract (WFE). Dried flowers of the plant were subjected to soxhlation using 80% methanol. The extract was complexed with soyalecithin in various drug:lipid ratios of 1:1, 1:2, 1:3, 1:4 and 1:5. Phytosomal complex of WFE was prepared by Ethanol method and Reflux method. All formulations were evaluated for vesicle formation, % yield, Entrapment efficiency and invitro drug release studies. Drug: lipid ratio of 1:3(E3) was suitable as it resulted in greater Entrapment efficiency of 89.15% & invitro drug release of 84.06%. Characterization of phytosomal complex (E3) revealed vesicle size and Zeta potential to be 213.1nm and 31.5mv indicating stability of the complex. Phytosomal gels of WPC E3 were formulated using different polymers (Carbopol 934, HPMC K4M, HPMC K100) in various concentrations. All gels were evaluated for homogeneity, pH, viscosity, drug content and invitro drug release studies. In vitro antioxidant activity of extract, phytosome and gel are in following order E3>PCG1>WFE. The data obtained in this study revealed that the objective has been met. Results of comparative study indicated that better solubility, increased antioxidant activity& in vitro drug release of phytosomal gel over crude extract.

Keywords: Woodfordia fruticosa, phytsomal gel, antioxidant activity.

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