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Abstract

MOLECULAR TYPING OF THE BACTERIA CLOSTRIDIUM DIFFICILE STRAINS ISOLATED FROM PATIENTS WITH ACUTE INTESTINAL INFECTION RIBOTYPING-PCR TECHNIQUE

Elnaz Nafisi*

ABSTRACT

Clostridium difficile infection is common in hospitals growing problem in recent years-have been reported. Detection of Clostridium difficile infection source control and prevent the spread of nosocomial infection caused by it is very important. Since PCR ribotyping method recently as an effective method to study the epidemiology of Clostridium difficile strains has been suggested, this study was conducted. Methods: A descriptive study was conducted over a 12 month period. 17 samples of patients suspected of infection with Clostridium difficile Clostridium difficile were isolated. All stool samples were treated with shock alcohol and yeast extract medium. The suspension of the broth treated Cephalexin cycloserine fructose agar (CCFA) enriched with 5% sheep blood culture method is linear up to 5 days were incubated in anaerobic conditions. The prevalence of isolates to determine the actual rate (Confidence Interval) of the population was estimated. Definitive identification, cdd-3 gene and determine the toxin profile of genes tcdA, tcdB were identified using PCR method. Clostridium difficile to identify Ribotype on samples obtained from hospitalized patients were PCR using primers specific ribosomal genes. Results: Of 89 samples, 17 (7/15%) samples were positive. The frequency of isolates with profiles toxin A + B + 12 (59/70%), A + B- 1 (9/5%) and A-B + 4 (9/23%) were reported. Ribotyping analyzed isolates showed that all of them have a different banding pattern. Conclusion: It seems that Ribotypes circulating varies in different parts of hospital infection may be due to endogenous or business strains pathogenic strains of the environment.

Keywords: Clostridium difficile bacteria, toxins, techniques Ribotyping-PCR.


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