Photo Gallery

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from  7.454 to 7.632  due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • WJPPS OCTOBER ISSUE PUBLISHED
  • OCTOBER 2020 Issue has been successfully launched on 1 October 2020.

Abstract

ASSESSMENT OF JAK2V617F MUTATION FREQUENCY IN PATIENTS WITH POLYCYTHEMIA VERA CITY OF TABRIZ IN IRAN

Shahin Asadi*, Elham Alizadeh Milani, Ali Nazirzadeh, Vahid Ghorbani

ABSTRACT

JAK2V617F mutation in the diagnosis and classification of myeloproliferative neoplasms has found wide application. In this study, JAK2V617F mutation frequency in patients with polycythemia vera Tabriz city were investigated. In this study, blood samples from 87 patients with polycythemia vera JAK2V617F mutation was investigated. DNA samples with proteinase k obtained from peripheral blood was extracted from buffy coat. After quality control, to assess the JAK2 mutation status of ARMS-PCR polymerase chain reaction with allele-specific primers were used. PCR products were electrophoresed on polyacrylamide gel electrophoresis and ethidium bromide staining were evaluated. Results: JAK2V617F mutation in 78.3% (58 patients) was observed myeloproliferative neoplasms. 58 patients with polycythemia vera, essential thrombocythemia, and 11 of 18 patients with primary myelofibrosis. Conclusion: This study showed that JAK2V617F mutation using allele-specific AST in patients with myeloproliferative neoplasms, speed and accuracy of diagnosis and the subsequent fate of patients with neoplasms Myeloproliferative promotes.

Keywords: myeloproliferative neoplasms, mutation JAK2V617F, reaction ARMS-PCR.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More