Abstract

NEW SIMPLE SPECTROPHOTOMETRIC AND DENSITOMETRIC METHODS FOR DETERMINATION OF ATORVASTATIN IN PRESENCE OF ASPIRIN IN PURE FORM AND PHARMACEUTICAL DOSAGE FORM.

Khalid A. M. Attia, Mohamed W.I. Nassar and Ashraf Abdel-Fattah*

ABSTRACT

Four, simple, accurate, selective and sensitive spectrophotometric and densitometric methods were developed for the determination of atorvastatin(ATV) in presence of Aspirin (ASP) without previous separation. Method A, Bivariate method using optimum wavelengths (240 and 250 nm) which formed by Kaiser’s method, with mean percentage recovery of 100.10±0.711. Method B, Area under curve method using two wavelength regions (2 5 225 nm) and (2 5 245 nm) with mean percentage recovery of 100.54±0.318. Method C, Q-analysis method where 277 nm is the iso-absorptive point for both the drugs and 246 nm which is λ max of atorvastatin with mean percentage recovery of 99.31±0.945. Method D, TLC-densitometry, separation was carried out on silica gel plates using methanol and chloroform (6:4 v/v). ATV and ASP were resolved with Rf values of 0.73 and 0.32 respectively. Determination was carried out at 246nm with mean percentage recovery of 100.04±0.782. Results were statistically compared to a reported method and no significant difference was noticed regarding accuracy and precision.

Keywords: Bivariate; Area under curve; Q-analysis; Densitometric methods.