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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

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WJPPS: APRIL ISSUE PUBLISHED

April Issue has been successfully launched on 1 April 2024.

Abstract

EXTRACTION, PURIFICATION AND CHARACTERIZATION OF EXO-POLYGALACTURONASE FROM CUCURBITA PEPO A PUMPKIN VARIETY COLA LAMBGOL SAMRAT 2

Pranjali Borkar*, Sushil Mhaske and Dr. Anil Kumar

ABSTRACT

Polygalacturonase (PG) is the most studied cell wall hydrolase. Germinating seeds of Cucurbita pepo, the pumpkin variety cola lambgol samrat 2 exhibited polygalacturonase (EC.3.2.1.67) activity. The maximal polygalacturonase activity was observed on the fourth day of germination. Enzyme was found to be in multiple forms, which was partially purified using the techniques of (NH4)2SO4 fractionation, DEAE-cellulose chromatography and sephadex G-200 gel filtration chromatography. Paper chromatographic results shows that the enzyme is an Exo-polygalacturonase. The maximum activity of the enzyme was found at pH 4.5. The enzyme exhibited optimum temperature of 64 0C.

Keywords: Exo-polygalacturonase, Cucurbita pepo, (NH4)2SO4 fractionation, DEAE-cellulose chromatography, sephadex G-200 gel filtration chromatography. Paper chromatography.

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