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Abstract

BIOSYNTHESIS AND IN VITRO ANTHELMINTIC ACTIVITY OF SILVER NANOPARTICLES USING AQUEOUS LEAF EXTRACTS OF AZADIRACHTA INDICA

S. Priya* and S. Santhi

ABSTRACT

The possibility of using plants in the deliberate synthesis of nanoparticles is a recent phenomenon. Preliminary investigations were carried out to assess the vermifugal and vermicidal actvity of biological silver nanoparticles in-vitro. Albendazole were used as standard reference drug and distilled water as control. Various concentrations of silver nanoparticles and aqueous extract of Azadirachta indica were tested as per standard protocols. It was found that anthelmintic activity of colloidal solution of silver nanoparticles was more than the aqueous extract. Overall the anthelmintic activity revealed the concentration-dependent nature of the aqueous extracts and colloidal solution of silver nanoparticles. Different concentration of aqueous extracts and colloidal solution of silver nanoparticles were tested against adult earthworms Eudrilus eugeniae and Eisenia fetida
as test worms. The bioassay involved determination of the time of paralysis and time of death neem leaf assisted green synthesized exhibit significant anthelminthic activity as compared to standard.

Keywords: Azadirachta indica, silver nanoparticles, aqueous extract, Eudrilus eugeniae, Eisenia fetida, anthelmintic activity.


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