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Abstract

PHYTOCHEMISTRY, GC-MS ANALYSIS AND INVITRO CYTOTOXIC ACTIVITY OF PRUNUS ANGUSTIFOLIA LEAVES AGAINST MCF-7 BREAST CANCER CELL LINE

*Poongodi T., Srikanth R. and Lalitha G

ABSTRACT

The current investigation was carried out to study the preliminary phytochemical screening, GC-MS and In vitro cytotoxicity assay of leaf extract of Prunus angustifolia. Phytochemical screening revealed the presence of active plant metabolites like alkaloids, phenol, tannins, glycosides, saponins and diterpenes. It was further continued to identify 8 different compounds of medicinal use in the Prunus angustifolia by GC-MS analysis. The study also investigated about the Invitro cytotoxic nature of Prunus angustifolia leaves in breast cancer cell line (MCF-7) using MTT assay. IC50 value was found to be 99.97μg/ml. 100% cell inhibition occurred at 300 μg/ml.

Keywords: Prunus angustifolia, MCF-7 cell line, GC-MS analysis, Cytotoxicity, MTT assay.


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