Abstract

BIOSYNTHESIS OF SILVER NANOPARTICLES BY GANODERMA APPLANATUM, EVALUATION OF THEIR ANTIBACTERIAL AND ANTIBIOTIC ACTIVITY ENHANCING POTENTIAL

*Deepak K. Rahi and Madhurika Barwal

ABSTRACT

The synthesis of various metal nanoparticles is now the most active area of research in nanotechnology. There are many chemical as well as physical methods, for the synthesis of metal nanoparticles however, green synthesis or biosynthesis involving different biological agents is the most emerging and preferred method of synthesis due to various reasons. In present communication we are reporting the synthesis of silver nanoparticles (AgNPs) using mushroom Ganoderma applanatum. The synthesized AgNPs have been characterized by UV/Vis spectroscopy, transmission electron microscopy (TEM), and Fourier Transform Infrared (FTIR) spectroscopy. The synthesized AgNPs were found to be in range of 4-22 nm as confirmed by TEM. FTIR analysis revealed the presence of proteins, amino acids, aldehydes, alcohol and carboxylic acids responsible for the reduction, stabilization and capping of AgNPs. The silver nanoparticles were found to possess remarkable antibacterial activity against standard test pathogenic strains, namely Methicillin- resistance Staphylococcus aureus (MRSA), Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. Further, AgNPs were also evaluated for their efficacy to enhance the antibiotic activities of some important broad range commercial antibiotics (Methicillin, Penicillin, Amoxycillin and Ampicillin) and the result obtained revealed a manifold increase of antibacterial activity against the test pathogens.

Keywords: Ganoderma, Amoxycillin, aureus, aeruginosa, AgNPs.