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Abstract

A VALIDATED REVERSE PHASE HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF LAMIVUDINE,STAVUDINE AND NEVIRAPINE IN PHARMACEUTICAL DOSAGE FORMS

P.Satyanarayana*, K.L.N.N.S.V.K.Pavankumar, A.Srinivasa Rao, G. Subrahmanya Sastry

ABSTRACT

A new, rapid, simple, precise and accurate reverse-phase HPLC method was developed and validated for the simultaneous determination of Lamivudine,Stavudine and Nevirapine in commercial tablets. The method has shown adequate separation for Lamivudine,Stavudine and Nevirapine. Separation was achieved on Zorbax C8 (250 mm × 4.6mm; 5 µm) column using isocratic method with 0.1%OPA:Acetonitrile(50:50) system at room temperature and the detection was carried out at 290 nm using photodiode array (PDA) detector. The linearity of the proposed method was investigated in the range of 37.5-112.5μg/ml (r2=0.999), 7.5-22.5μg/ml (r2=0.999), 50-150μg/ml (r2=0.999) for Lamivudine,Stavudine and Nevirapine respectively. The limit of detection (LOD) was 0.146,0.1705 and1.215 for Lamivudine, Stavudine and Nevirapine respectively. The limit of quantification (LOQ) was 0.486,0.5682 and 4.049 for Lamivudine,Stavudine and Nevirapine respectively. The relative standard deviation (RSD) of six replicates is less than 2%. This HPLC method is applied successfully to the simultaneous quantitative analysis of Lamivudine, Stavudine and Nevirapine in commercial tablets.

Keywords: RP-HPLC, Lamivudine,Stavudine and Nevirapine.


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