Abstract

QUANTITATIVE DETERMINATION OF DRUGS IN BULK AND PHARMACEUTICAL DOSAGE FORMS BY USING 2,3-DICHLORO-5,6-DICYANOBENZOQUINONE

Bathini Srinivas, P. Yadagiriswamy and G. Venkateshwarlu*

ABSTRACT

The present work narrates determination of drugs viz,. Metaxalone (MTX), Rasagiline (RSG), Aprepitant (APR) and Linezolid (LZD) based on formation of Charge Transfer Complex of drugs as n-electron donor with 2,3-dichloro-5,6-dicyanobenzoquinone(DDQ) as π-acceptor in bulk and pharmaceutical dosage forms. The selected drugs turned the yellow color solution of DDQ in Acetonitrile to thick brown color and exhibited a band at 585nm due to the formation of Complex of drugs with DDQ. Under the optimized experimental conditions, Beer, s law is obeyed over the concentration ranges of 15-75 μg/ml, 10-50 μg/ml, 5-25 μg/ml and 10-50 μg/ml for, MTX, RSG, APR and LZD respectively. The sensitivity, accuracy of methods, effect of reagent concentrations, polarity of solvents and effect of reaction time have been studied and optimized. The stoichiometric relationship was determined by Job’s continuous method and is found to be 1:1 in each case. These methods have been validated in terms of ICH guidelines and applied to the quantification of selected drugs in bulk and dosage forms.

Keywords: Drugs, 2,3-dichloro-5,6-dicyanobenzoquinone,Charge-transfer complex, Quantification and Validation.