Abstract

PHYTOCHEMICAL SCREENING, CHARACTERIZATION AND BIOLOGICAL ACTIVITIES OF PETROSELINUM CRISPUM (PARSLEY) LEAF OIL

Tirhas Mulugeta*, C.R Unnithan and Desta Tesfay

ABSTRACT

The essential oil of parsley (Petroselinum crispum (mill) Fuss) obtained by hydro distillation was characterized by its physicochemical properties, phytochemical screening and gas chromatography-mass spectrometry (GC-MS) profiles. The major components of theessential oil were 1, 3, 8-para-menthatriene, 1-methyl, 4-isopropenyl benzene and 4, 7- dimethoxy-5-prop-2-enyl-1, 3-benzodioxole. The average oil yield was 0.6 ± 0.29% (w/w) with yellowcolor and pleasant odout. The essential oil showed a high antimicrobial activity towards staphylococcus aureus and salmonella typhimurium and average effectiveness against Escherichia coli. The key effect of parsley in the growth of lactobacillus delbrueckii was studied. The results showed that lactobacillusdelbrueckiiproduced high concentration of biomass on the added of leaf oils, when compare with the control, characterized by the addition of different concentration (0.2, 0.1 and 0.05) g/mL of the essential oil in the growth exponential phase.

Keywords: Petroselinum Crispum (Parsley), Essential oil, GC-MS, Antimicrobial activity, Lactobacillus delbrueckii.