OPTIMIZATION OF MEDIUM COMPOSITION FOR BIOMASS PRODUCTION OF NEOLENTINUS KAUFFMANII USING RESPONSE SURFACE METHODOLOGY
Johnsy. G* and V. Kaviyarasan
1Center for Advanced studies in Botany, University of Madras, Maraimalai campus, Chennai, Tamilnadu, India.
ABSTRACT
The optimization of submerged culture conditions and nutritional
requirements was studied for the production of biomass from
Neolentinus kauffmanii using the statistically based experimental
design in a shake flask culture. Maximum mycelial biomass was
observed at 25ºC. The optimal initial pH for the production of mycelial
biomass was found to be pH4.0 and pH6.0, respectively. The
coefficient of determination (R2) for the model was 98.5%. Probability
value (P<0.0001) demonstrated a very high significance for the
regression model. By solving the regression equation and also by
analyzing the response surface counter plots, the optimal process
parameters were determined, fermentation temperature, fermentation
time and inoculum volume. Under the optimal conditions the
corresponding response value predicted for biomass production was
328 mg/cm3 which were confirmed by validation experiments.
Keywords: Response surface methodology, Neolentinus kauffmanii, biomass, probability value, regression.
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