Abstract

MICROPROPAGATION OF PHYLLANTHUS AMARUS FROM NODAL EXPLANT

Dr. H. David Raja*

ABSTRACT

A simple micropropagation method is reported for Phyllanthus amarus one of important hepoto-protective medicinal plant from nodal explant. High frequency of microshoots were obtained from MS basal medium supplemented with various concentrations of cytokinins (BAP 1.0 - 4.0 mg/l and KIN 1.0 - 4.0 mg/l and 2ip 1.0 - 4.0 mg/l). Maximum numbers of shoot production were obtained from nodal explants in 3.0mg/l BAP medium. The root induction was carried out by using MS basal medium without adding of any auxins. The rooted plantlets were hardened and transferred to the field. The transplantation survival rate was 90 – 95% after 25 days.

Keywords: Micropropagation, Phyllanthus amarus, Node, Multiple shoots.