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Y.Samatha*, A.Srividya, A.Ajitha and V.Uma Maheswara Rao


UPLC technique a enhancement in speedy resolution as well as the sensitivity of analysis by using particle size less than 2 μm, operates at higher pressure, while the mobile phase runs at greater linear velocities as when compared to HPLC. It offers an advantage of time saving & reduces solvent consumption. Now a day’s substantial technological advances have been done in enhancing particle chemistry performance, improving detector design, in optimizing the system, data processors and various controls of chromatographic techniques. This review focuses on the basic principle, instrumentation of UPLC and its advantages over HPLC, furthermore emphasizes on various pharmaceutical applications of UPLC technique.

Keywords: ultra performance liquid chromatography, small particle size, High pressure.

[Full Text Article]

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