Abstract

TUNABLE SYNTHESIS OF GELATIN NANOPARTICLES EMPLOYING SOPHOROLIPID AND PLANT EXTRACT, A PROMISING DRUG CARRIER

Zeinab Ghasemishahrestani, Mihir Mehta, Priti Darne, Aishwarya Yadav, Asmita Prabhune*, Suvidya Ranade*.

ABSTRACT

Biodegradable polymer nanoparticles are being explored as drug delivery vehicle for various diseases till date. We report for the first time the green synthesis of tunable gelatin nanoparticles by nanoemulsion method using Cassia fistula leaf extract and Sophorolipids synthesized by a non-pathogenic yeast Candida bombicola ATCC 22214 a glycolipid biosurfactant. Sophorolipid and plant extract helped in compaction and reduction in size of gelatin nanoparticles with effective loading of Quercetin so as to get spherical nanoparticles with size ranging between 20-60 nm. SEM, TEM and DLS results are in agreement for the size of nanoparticles. Sustained
drug release pattern was seen in vitro at pH 7.4. These drug loaded nanoparticles were further characterized and tested for cytotoxicity on MCF7 cancer cell line. The bare and drug loaded nanoparticles displayed differential cytotoxicity towards MCF7 cancer cells. These biogenic nanoparticles are biocompatible and found to be good candidates for sustained drug delivery in diseases like cancer.

Keywords: Biodegradable, Gelatin, Cassia fistula, Sophorolipids, Quercetin, Cytotoxicity.