Abstract

PHYSICO-CHEMICAL AND MICROBIOLOGICAL STANDARDIZATION OF SUFOOFE SAILAN: A UNANI POLYHERBAL FORMULATION

Seema Rani*, Khaleequr Rahman, Mohd. Younis, Sadiya Noorul Basar

ABSTRACT

Sufoofe sailan (SS) is a Unani herbal formulation used to treat sailanur rehm (leucorrhoea) since ancient time. Though, SS is very efficacious to treat this condition, its standardisation is not carried out till date. Keeping this goal in mind some standards for SS using scientific analytical procedures was prepared and finished product was evaluated for various organoleptic and physicochemical parameters. Finished product was light brown (7.5YR5/6-Panton colour chart), odourless and pleasantly sweet in taste. The physicochemical parameters expressed as mean values of the three readings calculated showed loss of weight on drying, total ash, acid insoluble ash, water soluble ash as 4.7±0.03%, 2.57±0.02%, 1.19±0.00% and.77±0.01% respectively. The mean values of bulk density, tapped density, hausner’s ratio, compressibility index were 0.48±0.01gm/ml, 0.63±0.00gm/ml, 1.29±0.01 and 23±1.0% respectively. pH of 1% and 10% solution were 4.76±0.00 and 5.23±0.00 respectively. Extractive values in chloroform, petroleum ether, alcohol and aqueous solvents by nonsuccessive extraction method were 1.19±0.02%, 0.45±0.02%, 10.3±0.04%, 22.18±0.09% respectively. Qualitative analysis showed presence of all major organic constituents except saponins and steroids. In inorganic one calcium was present and magnesium, copper and iron were absent. Quantitative analysis for total alkaloids, total glycoside, and total tannins were 2.58±0.00%, 0.72±0.01% and 10.39±0.03% respectively. Desitometric HPTLC analysis was also carried out to develop finger printing of SS. Microbiological analysis showed total bacterial count 3×104cfu/g, total fungal count 20cfu/g and specific pathogens were absent. As physico-chemical profile of SS is not reported till date this data could be taken as standard for quality control purpose.

Keywords: HPTLC, Leucorrhoea, Standardisation, Sufoofe sailan.