Abstract

ENHANCED EFFICACY OF KETOCONAZOLE COATED SILVER NANOPARTICLES AGAINST THE FUNGUS MALASSEZIA FURFUR A DANDRUFF CAUSING AGENT

Jasmine Mathew, Vandana Rathod*, Dattu Singh, Shivaraj Ninganagouda, Ashish Kumar Singh and Prema Kulkarni

ABSTRACT

The present study describes simple and effective method of silver nanoparticles (AgNp‟s) synthesis via green root using the fungus Aspergillus pseudodeflectus. The synthesized AgNp‟s were characterized by visual observation, UV-Spectroscopy which showed maximum absorption at 422.5nm, FTIR showed bands at 3418.59, 1636.52, 1384.27, 1075.84cm-1, TEM revealed the formation of spherical nanoparticles with size ranging between 20-60nm, SEM showed the formation of well dispersed AgNp‟s, XRD revealed the crystalline nature of AgNp‟s, EDS showed the optical absorption peak at 3kev, which confirmed the presence of elemental silver. The efficacy of AgNp‟s against dandruff causing Malassezia furfur (Anti-dandruff activity) was performed. The disc diffusion method and In Vitro activity of Ketoconazole alone, AgNp‟s and Ketoconazole coated AgNp‟s was done. The zone of inhibition for Ketoconazole alone was 22 mm, while that of AgNp‟s alone was only 18mm. Surprisingly we could observe the zone of inhibition of 30mm which was enhanced to a maximum extent when Ketoconazole was coated with AgNp‟s produced by the fungus A. pseudodeflectus. The minimal inhibitory concentration was found to be 0.062 for Ketoconazole and 0.025 for AgNp‟s alone, where as it was 0.0108 for Ketoconazole coated AgNp‟s which clearly reveals the enhanced efficacy of AgNp‟s with minimum concentration.

Keywords: Aspergillus pseudodeflectus, Silver Nanoparticles, TEM , SEM, FTIR, XRD, EDS, Anti dandruff.