Abstract

SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF FURAZOLIDONE IN PHARMACEUTICAL FORMULATIONS AND HUMAN BLOOD SAMPLES WITH BPB

B. Ramachandra, K. Sivarami Reddy, Ch. Giridhar, K. Vinod Kumar, N.Venkatasubba Naidu*

ABSTRACT

A simple, sensitive, selective rapid spectrophotometric method has been developed for the determination of synthetic antimicrobial furazolidone in pure form and pharmaceutical formulations based on the oxidative coupling reaction with BPB reagent, at PH_3.6 which is extractable at 410 nm. Beer’s law is obeyed in the concentration ranges 5-30 μg ml-1. And 3-18 μg ml-1. For blood sample the developed method was applied directly and easily for the analysis of the Pharmaceutical formulations and blood sample. R.S.D was found to be 0.07564% to 0.14587 and recovery was found between 99.33 % to 99.97% respectively. The method was completely validated and proven to be rugged. The interferences of the other ingredients and excipients were not observed. The repeatability and the performance of the proved method were established by point and internal hypothesis and through recovery studies.

Keywords: Spectrophotometry, Furazolidone, Blood Sample & BPB / HC/ Chloroform.