Abstract

EFFECT OF PHYLLANTHUS NIRURI EXTRACTS ON COLONY FORMING UNITS OF THE GRANULOCYTE - MACROPHAGE SERIES ACTIVITY IN SERUM OF MICE

P. Veeresh Kumar*, Mangilal. T, Satish Kumar. M, Naga Kishore. R

ABSTRACT

This study was performed to elucidate the possible mechanism of action of Phyllanthus Niruri In view of the fact that Phyllanthus niruri induced leucocytosis. Ten to twelve-week-old either sex mice (Swiss albino) weighing 18 to 22 g were selected for the study. On the 11th day, the animals were sacrificed and blood was collected from the heart. Total and differential white blood cell count was performed. Serum was separated and stored at 80 ºC. This was used for colony stimulating activity. The results were expressed as the mean  S.D. for the indicated number of experiments. Based on the results we found that the dose dependent increment was observed in the concentration of DLC. It might be due to CFU-GM stimulating activity of Phyllanthus niruri extract. GM-CSF stimulates stem cells to produce granulocytes and Monocytes. On account of this fact, we concluded that the neutrophil activity stimulated in the extract treated group which is helpful for its immunostimulant potential.

Keywords: Colony-Stimulating Factors (CSFs), colony forming unit (CFU) and Phyllanthus niruri.