Abstract

ELECTROPHORETIC STUDY OF THE ANTAGONISTIC EFFECT OF SALICIN ISOLATED FROM EGYPTIAN WILLOW LEAVES (SALIX SUBSERRATA) AGAINST THE EFFECT OF GAMMA IRRADIATION IN MALE RATS

Hayat M. Sharada, Mohga S. Abdalla, Ibrahim A. Ibrahim, Monira A. Abd El Kader and *Wael M. Kamel

ABSTRACT

The study aimed to study efficiency of salicin to ameliorate irradiation effect on various electrophoretic protein and zymogram patterns in rats. Materials and Methods: The polyacrylamide gel electrophoresis for native protein, lipoprotein and zymogram (esterase, catalase and peroxidase) were carried out in serum samples of all groups. Results: Irradiation caused various abnormalities in all electrophoretic patterns (protein, lipoprotein and zymogram). It caused qualitative alterations represented by disappearance of some or all normal bands with appearance of abnormal bands and /or deviation of normal bands to be appeared with another data (Rfs, Mwts and B % values). It caused quantitative alterations represented by changing B % of the bands appeared with normal Rf and Mwts. For electrophoretic protein pattern, salicin administration reduced the irradiation effect on serum sample of the irradiated salicin post-treated group (SI = 0.70) and for lipoprotein pattern, salicin decreased the irradiation effect in irradiated salicin pre-treated group (SI = 0.80). While in the electrophoretic zymogram, salicin administration showed no antagonestic effect against the qualitative and quantitative mutagenic effect of irradiation in all irradiated salicin treated groups. Conclusion: The results showed that salicin prevented the irradiation effect on electrophoretic protein pattern in the irradiated salicin post-treated group and lipoprotein pattern of irradiated salicin pre-treated group. While in the electrophoretic zymogram, salicin could not prevent the irradiation effect in all irradiated salicin treated groups.

Keywords: Gamma rays, Rats, Protein pattern, Lipoprotein, Enzyme electrophoresis.