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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2020
Citation	6651	4087
h-index	26	21
i10-index	174	83

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WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

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WJPPS: NOVEMBER ISSUE PUBLISHED

NOVEMBER 2025 Issue has been successfully launched on 1 NOVEMBER 2025.

Abstract

NEW SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF THIOCOLCHICOSIDE IN BULK AND PHARMACEUTICAL FORMULATIONS

*Chinna Raja.Pydi, Nirmala Jyothi.P, Pallavi.P, Rafath Sulthana.Md, Kiran Kumar.G

ABSTRACT

Three simple spetrophotometric methods (A, B and C) have been described for the assay of Thiocolchicoside in bulk and in pharmaceutical formulations. In method A Thiocolchicoside is condensed with P-dimethylaminobenzaldehyde (PDAB) to form a yellow coloured Schiff’s base having an absorption maximum (λmax) of 400nm. Method B is based on the oxidation / reduction reaction between the Thiocolchicoside and hydrochloride reagent to form a yellow coloured chromogen with λmax at 400 nm. Method C involves oxidation of Thiocolchicoside with ferric chloride followed by coupling with N-methylbenzothiazolone hydrazone (MBTH) to form yellowish green coloured complex with λmax at 410nm.These methods have been statistically evaluated and are found to be precise and accurate.

Keywords: Thiocolchicoside, MBTH, Validation, UV-Visible.

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