Abstract

SYNTHESIS AND ANTI-MICROBIAL AGENTS OF NOVEL (E)-N'-4, 4’-DIFLUORO-CYCLOHEXANECARBOXYLIC ACID (SUBSTITUTED-BENZYLIDENE)-HYDRAZIDE DERIVATIVES

Tella.Lakshmi Viveka, Mariyam Saba, S.N.T.Sunitha, Y.Aparna, L.Nalanda Sharada*

ABSTRACT

A series of (E)-N'-(substituted-benzylidene)cyclohexanehydrazide derivatives were synthesized by coupling of 4,4'-diflouro cyclohexane carboxylic acid hydrazide with different aromatic aldehydes in the presence of ammonium acetate, acetic acid in catalytic amount, methanol as solvent at room temperature. All the synthesized compounds were confirmed and characterized by using various spectral technique like IR, H1NMR, C13NMR, and mass spectral studies. All the synthesized hydrazone derivatives were screened for anti-bacterial activities by disc diffusion methodagainst Staphylococcusareus,Bacillusmegaterium (Gram+ve);E.coli and Pseudomonasaeruginosa(Gram-ve)bacrerial strains,andantifungalactivity against Candidaalbicans, Rizopusmicrosporus var. oligosporus. The activity studies of all the synthesized compounds against bacterial strains in both gram positive and gram negative activity against bacterial strains revealed that the compounds 5a,5c,5d,5f,5g,5h,5i,5j,5k,5l and 5oshows moderate activity against Pseudomonas aeruginosa MTCC No. 1034compared with standard pencillin.off all the above compounds 5g and 5k have highest activity compared to all the synthesized compounds in gram negative in 100μg/ml/mm concentration. Activity against Fungi Rizopusmicrosporus var. oligosporus (MTCC No: 2785) revealed that the compounds 5a,5h,5j and 5n have highest activity compared to all the synthesized compounds in 100μg/ml/mm concentration. This study proved that 4,4'difluoro cyclohexane hydrazone substituted benzilidenes synthesized by condencing 4,4'-diflouro cyclohexane carboxylic acid hydrazide with different aromatic aldehydes displayed moderate to potent activity.

Keywords: N-substituted benzylidene derivatives, cyclohexane hydrazide, anti bacterial activity, anti fungal activity.