Abstract

SCREENING AND EVALUATION OF BIOACTIVE COMPONENTS OF HEDYCHIUM CORONARIUM J. KOENIG IN NATURE GROWN AND IN VITRO REGENERATED PLANTS BY GC-MS ANALYSIS

Manju Verma and Yogendra Kumar Bansal*

ABSTRACT

About 80% of the World’s population depends on the traditional medicines for various ailments. The yield of steam distillation of Hedychium coronarium essential oils and its physicochemical constants were determined. The GC-MS analysis of nature grown and in vitro grown plant samples were carried out using GC-MS-QP 2010 Plus model with methanol as solvent. Several oil components were identified based upon comparison of their mass spectral data with those of reference compounds published in literature or stored in a computer library. The oil was found to contain monoterpenes, sesqueterpenes and diterpenes. The major components of the oil were 2-methoxy-4-vinylphenol (15.86%), .beta.-d-glucopyranoside, methyl (53.06%), hexadecanoic acid (7.11%), stigmast-5-en-3-ol, (3.beta.)- (12.71%), 2-hydroxy-gamma-butyrolactone (9.42%), 2-oxabicyclo[2.2.2]octan-6-ol, 1,3,3-trimethyl- (2.91%).

Keywords: Bioactive compounds, GC-MS analysis, Medicinal plant.