Abstract

ANTIOXIDANT POTENTIALS OF ACTIVE PRINCIPLES FROM TERMINALIA BELLARICA FRUIT – AN IN-VITRO AND IN-VIVO APPROACH.

Manikkam Rajalakshmi* and Mary Selesty Sales

ABSTRACT

Introduction: Terminalia bellarica (T. bellarica) is a potential medicinal herb used from the ancient times to treat human diseases and disorders. The present study was designed to analyze the antioxidant efficacy of two active principles, octyl gallate (OG) and gallic acid (GA), isolated from the methanolic extract of T. bellarica fruit with reference to their action on free radicals, free radical scavengers using in vitro and in vivo approaches. Methods: Cells were cultured and incubated with OG & GA for 24 h at 37°C. The viability of the cells was measured using MTT assay. DMBA induced female Sprague- Dawley rats were used as animal models. Lipid peroxidation (TBARS), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) levels were determined with and without OG & GA treatment in both cell lines and breast and liver tissues. Results: Cell viability analysis provided 40 μM OG and 80 μM GA as IC50 concentrations of the compounds for both the cell lines. The lipid peroxidation levels estimated showed an increase in cancer groups and a significant decrease upon OG and GA treatment and a decrease in SOD, CAT and GPx levels in cancer groups which was found to be increased with OG and GA treatment. The above condition was noted in MCF-7, MDA-MB-231 cell lines and in breast and liver tissues of in-vivo models. Conclusion: The results obtained provides an insight for the anticancer and antioxidant potentials of OG and GA from T. bellarica on breast cancer models indicating its efficacy to be used for breast cancer therapies with further exploration on their mechanism of action.

Keywords: Phytotherapy, antioxidant, apoptosis, free radicals, scavengers.