Abstract

IMPROVED EFFICACY OF GLICLAZIDE WITH POLYHERBAL FORMULATION ON PHARMACODYNAMIC AND PHARMACOKINETIC ACTIVITY IN NORMAL RABBITS

M. Ganga Raju*, S. Satyanarayana and Eswar Kumar

ABSTRACT

The present study was carried out to evaluate the polyherbal formulation (PHF) on pharmacodynamic and pharmacokinetics of gliclazide in normal rabbits. A few aqueous extracts of Vinca rosea, Gymnema sylvestre, Tinospora cordifolia, and Emblica officinalis were selected and tested for hypoglycemic and anti hyperglycemic activity on normal and alloxan induced diabetic rats. The optimal doses of the above selected aqueous extracts of Vinca rosea, Gymnema sylvestre, Tinospora cordifolia, and Emblica officinalis were found to be 100, 100, 200 and 300 mg/kg bd.wt. so a polyherbal formulation (PHF) was prepared in the ratio of 1:1:2:3 respectively. In rats the polyherbal formulation was evaluated at three doses 75, 150 and 300 mg/kg bd.wt. and the dose 150 mg/kg that produced an optimal reduction in blood glucose reduction was selected. The same dose is converted to rabbits and a dose of 117 mg/ 1.5 kg bd.wt was selected for the present study. Blood samples were collected by puncturing the marginal veins of rabbits at desired intervals of time and analyzed for blood glucose by GOD-POD method. Gliclazide a second generation sulfonyl urea was selected for the interaction study with that of polyherbal formulation. The human dose of gliclazide is 80 mg/kg bd.wt. Dose response relationship of gliclazide was established with 2.8, 5.6 and 11.2 mg/1.5 kg bd.wt and the dose of 5.6 mg/1.5 kg.bd.wt that produced optimal reduction (30-40%) in blood glucose levels was selected for the present study. The prior administration of polyherbal formulation significantly increased the hypoglycemic and antihyperglycemic activity of gliclazide.

Keywords: Polyherbal formulation (PHF), hypoglycaemic, antihyperglycemic activity, Gliclazide.