Abstract

EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY OF OVOUTOLINE LIQUID FORMULATION AGAINST THE DENATURATION OF PROTEIN

Rukhsar Iqbal Wasta*, Dibyangana Susanta Duttagupta, Dr. Varsha Milind Jadhav, Dr. Vilasrao J. Kadam

ABSTRACT

Objective: To evaluate the in vitro anti-inflammatory effect of Ovoutoline liquid formulation against the denaturation of proteins. Methods: The different concentrations of Ovoutoline liquid formulation was incubated with egg albumin in controlled experimental conditions and subjected to determination of absorbance to retrieve the anti- inflammatory property. Diclofenac sodium was used as the reference drug. Results: The presented findings exhibited a concentration dependent inhibition of protein (albumin) denaturation by the Ovoutoline liquid formulation. Conclusions: From the present study it can be concluded that Ovoutoline liquid formulation possessed marked in vitro anti – inflammatory effect against the denaturation of protein. The effect was reasonably due to the alkaloids, flavanoids and polyphenolic constituents present in the formulation.

Keywords: Menopause, Anti-inflammatory, Ovoutoline.