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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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April Issue has been successfully launched on 1 April 2024.

Abstract

A VALIDATED RP-HPLC METHOD FOR ESTIMATION OF EFAVIRENZ IN PHARMACEUTICAL FORMULATION

*S.Ramesh¹, S.Alexandar² and S.Muniyappan³

¹Department of pharmaceutical analysis, J.K.K.M. College of pharmacy, Kumarapalayam, Erode, India.

²Department of pharmaceutical chemistry, JSS College of Pharmacy Ooty-643001 India.

³Department of pharmaceutical Bio technology, Nandha College of pharmacy, Erode, India.

ABSTRACT

A simple, selective, rapid, precise and economical reverse phase high pressure liquid chromatographic method has been developed for the estimation of Efavirenz from pharmaceutical formulation. The method was carried out on a Princeton SPHER C18 (25 cm x 4.6 mm i.d., 5 μ) column with a mobile phase consisting of acetonitrile: potassium Dihydrogen ortho phosphate (adjusted to pH 3.5 using orthophosphoric acid) (50:50 v/v) at a flow rate of 1.0 ml/min. Detection was carried out at 254 nm. Theophylline was used as an internal standard.The retention time of Efavirenz and Theophylline was 2.45 and 3.10 min, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability.The proposed method can be used for the estimation of Efavirenz in pharmaceutical dosage form.

Keywords: Efavirenz, Pharmaceutical Formulation.

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