Abstract

THE EFFECT OF SUBCHRONIC LOW DOSE OF DDVP AND SODIUM AZIDE ON THE HAEMATOLOGICAL INDICES OF ALBINO RATS

Patrick Ugochukwu Agbasi*, Nse Abasi, Joy Jeremiah Onye, Chinasa Ibeawuchi, Samuel C Uzoechi, Emmanuel Ayoma Alagwu, Chukwubike U Okeke, Godwin C
Uloneme

ABSTRACT

Background: The frequent use of organophosphorous insecticides in our everyday life especially in our houses, offices and even against pests to preserve foods and agricultural products especially in the third world and sodium azide (a herbicide) extensive use in the farms have necessitated this study. Purpose: This research is designed to investigate the effect of low dose DDVP and sodium azide coadministration on hematological parameters of Albino rats. Method: Seven groups of five rats were used for the study. Group A was used as control and received no chemical treatment, groups B and C were injected with 1% and 3% LD50 of sodium azide respectively, while groups D and E received 1% and 3% LD50 of DDVP respectively. Equally, group F received combined doses of 1% DDVP and 1% Sodium azide while group G received 3% DDVP and 3% Sodium azide. The chemicals were administered at alternate days for twenty one days. At the end of twenty one days study period, the animals were anesthetized with chloroform and blood were aspirated through cardiac puncture into heparinized containers and then analyzed with hematology auto analyzer. Results: showed that the hemoglobin concentration (Hb), packed cell volume (PCV), red blood cell count (RBC), mean corpuscular haemoglobin concentration (MCHC) of rats in groups B and C were not statistically significantly affected compared to control while the total white cell count (TWCC) of group C, platelet, lymphocyte and neutrophil count for both groups B and C were statistically significantly reduced compared to control. For rats in groups D and E, the Hb, PCV, RBC count, TWCC, MCHC, platelet count, neutrophil and lymphocyte levels were significantly reduced compared to control. In both groups F and G, the values of TWCC, MCHC, neutrophil, lymphocyte and platelet count were significantly reduced compared to control. All the polymorphological leucocytes, red cell indices and platelets values in group F were dose dependently reduced compared to group G. Conclusion: Co- administration of DDVP and sodium azide produced severe reduction of polymorphological leucocytes, red cell indices and platelets

Keywords: DDVP, Sodium azide, Auto analyzer, Hematology parameters, Albino rats.