Abstract

COMPARATIVE STUDY OF ANTIMICROBIAL COMPOUNDS EXTRACTED FROM LEAVES OF NICOTIANA TABACUM AND CIGARETTE

Rahul Shivaji Patil*, Ashwini Bhimrao Desai and Simran Amogh Wagh

ABSTRACT

The present study deals with extraction and characterization of antimicrobial compounds from Nicotiana tabacum and cigarette. The extracts in organic solvents such as ethyl acetate and ethanol were prepared for the study. Ethyl acetate extracts of Nicotiana tabacum and cigarette showed presence of steroids, tannin and saponin. Ethanolic extract of both showed presence of diterpenes, steroids, flavonoids, tannin, coumarin, phenols and saponin while cigarette are rich in tannin and flavonoids. Antimicrobial activity of Nicotiana tabacum and cigarette extracts were tested against Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, Bacillus cereus, Penicillium chrysogenum and Aspergillus niger by agar well diffusion method. The ethyl acetate extract of both samples showed significant range of inhibitory effect against Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium and Bacillus cereus but the ethanolic extract is more effective. The extracts were further analyzed by TLC and GCMS which showed presence of bioactive compounds in the Nicotiana tabacum and cigarette. The ethyl acetate extract of Nicotiana tabacum and cigarette, also showed antifungal activity against Aspergillus niger and Penicillium chrysogenum along with ethanolic extract. Thus, these results confirm the presence of antibacterial and antifungal compounds in Nicotiana tabacum and cigarette while compounds in cigarette shows higher zone of inhibition hence both the samples can be used as a source of antibiotics.

Keywords: Nicotiana tabacum, Cigarette, Phytochemical Analysis, Antimicrobial.