ANTIMICROBIAL AND FREE RADICAL SCAVENGING ACTIVITY OF SELECTIVE MEDICINAL PLANTS COMBINATION
Joginder Singh Duhan*, Akansha Rana, Pardeep Kumar Sadh, Pooja Saharan, Surekha
ABSTRACT
The present study was aimed to evaluate antimicrobial and free radical scavenging activity of selective medicinal plants combination extracts. The antimicrobial efficacy of the combination was tested by agar well diffusion assay while free radical scavenging activity of the combination was assessed against 1, 1- diphenyl-2-picrylhydazyl (DPPH) and ferric reducing agent. Total phenolic content and phytochemical screening was also carried out to assess their effect on free radical scavenging activity of the plant combination. Out of four extracts of medicinal plant combination, methanol and ethanol extract exhibited maximum activity against S. typhi, S. aureus and B. cereus, respectively. Ethyl acetate extracts of the combination was also found
to be significantly effective against S. typhi, Shigella and B. cereus. The minimum inhibitory concentration (MIC) values ranging from 6.5 to 50.0μg/ml. The lowest MIC values were obtained against S. typhi followed by S. aureus and B. cereus, indicating the susceptibility of the strain for ethanol and methanol extracts of the combination. The efficiency of a few extracts was even higher than the standard antibiotic (ampicillin) against few microorganisms. The free radical scavenging activity (as evaluated by DPPH assay) of ethanol extract of plant combination at concentration of 100 μg/ml was found to be 72.02 % followed by methanol i.e. 51.80 % where as maximum metal chelating activity was observed in methanol (78.53 %) followed by ethanol extract (65.64 %) and ethyl acetate extract (61.26 %) respectively. IC50 value of ethanol extract of the combination was approximately equal to that of L-ascorbic acid showing the maximum inhibitory effect followed by methanol extract as evaluated by DPPH assay while the same was also higher in methanol extract evaluated by ferrous reducing assay. The study thus helps in designing a potentially active antimicrobial and antioxidative decoction from the plants combination.
Keywords: Antimicrobial activity, MIC, antioxidant, free radicals, medicinal plants combination.
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