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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2020
Citation	6651	4087
h-index	26	21
i10-index	174	83

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WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

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WJPPS: NOVEMBER ISSUE PUBLISHED

NOVEMBER 2025 Issue has been successfully launched on 1 NOVEMBER 2025.

Abstract

DEVELOPMENT OF HPTLC METHOD FOR ESTIMATION OF VASICINE IN HERBAL FORMULATIONS AND QUALITY STANDARDIZATION IN ASFRE12

P. Ravi Kumar*

ABSTRACT

Vasicine is one of the phytoconstituents present in Adhatoda vasica (L.)Nees (Family: Acanthaceae) have been used in Indian systems of Medicine for cough, bronchitis, bronchial asthma etc. In this present study an attempt has been made to develop a HPTLC method for quantitative estimation of Vasicine in different extracts (Alkaloid extract, Methanolic extract and 70% Methanolic extract) and in different samples of Asfre12[Laboratory sample (Alkaloid extract), Commercial sample (Alkaloid extract), Laboratory sample (70% Methanolic extract), and Commercial sample (70% Methanolic extract)]. In this the alkaloid extract of A.vasica sample and laboratory sample of alkaloid extract yielded more percentage of Vasicine. Quality control and standardization was carried out in the polyherbal formulation (PHF) Asfre12.

Keywords: Adhatoda vasica, Vasicine, HPTLC, PHF, Asfre12.

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