HPTLC FINGERPRINT PROFILE AND ANTIMICROBIAL ACTIVITY OF LEAVES OF ACHYRANTHES ASPERA LINN.
Aparna Saraf* and Aruna Samant
ABSTRACT
The present work deals with phytochemical and antibacterial screening of leaves of Achyranthes aspera Linn. Preliminary phytochemical screening was done and HPTLC studies were carried out. Methanol extracts of the leaves was developed in the mobile phase of Toluene: Chloroform : Methanol (4:4:1) using standard procedures and scanned under UV at 254 nm, 366 nm and visible light at 540 nm. The in vitro antibacterial activity was performed on extracts of leaf of Achyranthes aspera Linn in petroleum ether, chloroform, acetone, ethanol, methanol and water against multi drug resistance organisms such as Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and
Staphylococcus aureus. The organic extracts of leaves of the plant at a concentrations of 0.005%, 0.007% and 0.01% were taken and their activities were measured. The results revealed that all the extracts had variable degree of antibacterial activity. HPTLC fingerprint analysis of leaf extract of Achyranthes aspera Linn. can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker.
Keywords: Achyranthes aspera Linn., antibacterial activity, HPTLC.
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