Abstract

RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF DEFERIPRONE IN CAPSULE DOSAGE FORM.

Manzoor Ahmed, Vinod Kumar Jangade*, Satishkumar Shetty A, Vijayakrishna C. Aradhya, Kuppast I.J and Anil kumar S.M.

ABSTRACT

The objective of the current study was to develop a simple, accurate, precise and rapid RP-HPLC method and subsequently validate as per ICH guidelines for the determination of Deferiprone using mobile phase [mixture of Phosphate buffer pH-3.6 and methanol in the ratio of 20:80 v/v] as the solvent. The retention time of Deferiprone was found to be 5.404 at 280 nm. The linearity of the proposed method was investigated in the range of 10-50 μg/ml and regression was found to be (r2 = 0.9998). The method was statistically validated for its linearity, accuracy and precision. Both inter-day and intra-day variation was found to be showing less % RSD (Relative Standard Deviation) value indicating high grade of precision of the method.

Keywords: HPLC, Deferiprone, ICH guidelines.