WJPPS Citation

Login

Search

News & Updation

  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  
  • New Impact Factor
  • WJPPS Impact Factor has been Increased to 8.025 for Year 2024.

  • WJPPS: MARCH ISSUE PUBLISHED
  • March Issue has been successfully launched on 1 March 2024.

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • Scope Indexed
  • WJPPS is indexed in Scope Database based on the recommendation of the Content Selection Committee (CSC).

Abstract

OPTIMIZATION OF CELLULASE PRODUCED BY FUNGUS ISOLATED FROM WATER

Harjot Pal Kaur* and Deepti Joshi

ABSTRACT

Cellulases are a group of hydrolytic enzymes capable of degrading cellulose to the smaller glucose units and have a wide range of applications in textile industry, drug industry, detergent industry, food-feed industry, bioethanol production and pulp-paper industry. This study was designed to assess the cellulase production of fungus isolated from water. 5 fungal isolates obtained from water samples were identified by morphological and microscopic features. These 5 isolates were screened for cellulase production and cellulase production of two isolates that gave highest zone of hydrolysis (P. chrysogenum - zone 18mm and T. reesei – zone 12mm) was estimated by CMCase and FPase assay then compared with cellulase activity of respective standard strains. Isolate 2 belonging to P. chrysogenum showed more enzyme activity (0.552 U/ml) than the isolate 1 belonging to T. reesei (0.363 U/ml) and both the isolates showed greater enzyme activity than their respective standard strains. Optimization of process parameters (temperature, pH, incubation time, carbon and nitrogen source) was carried out for the isolates and respective standard strains to maximize enzyme yield. On optimization T. reesei showed maximum enzyme activity at temperature of 30°C, pH 4, 120 hours of incubation with sucrose as carbon source and yeast extract as nitrogen source whereas P. chrysogenum showed maximum cellulase production at temperature of 30°C, pH 5, incubation of 144 hours, CMC as carbon source and ammonium nitrate as nitrogen source. Results indicate that fungi belonging to T. reesei and P. chrysogenum effectively produced cellulase and its yield was increased by optimizing process parameters.

Keywords: Cellulase, Morphological, Microscopic, Sucrose, Yeast Extract


[Download Article]     [Download Certifiate]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More