Abstract

DETERMINATION OF TOTAL PHENOLS AND ANTIOXIDANT ACTIVITY IN AQUEOUS AND ETHANOLIC EXTRACTS FROM THE LEAVES OF LECANIODISCUS CUPANIOIDES PLANCH (SAPINDACEAE)

Traoré Zeinab*, YEO Sounta Oumar, SILUÉ Kalamourou, KOUAMÉ Djè Kouamé Wenceslas, et DOUMBIA Idrissa

ABSTRACT

The objective of this study was to determine the polyphenol content and evaluate the antioxidant activity of aqueous extracts (EAq) and 70% ethanolic extracts (EEth70%) from the leaves of Lecaniodiscus cupanioides PLANCH. (Sapindaceae), a plant used in the treatment of diabetes in the Man Department (western Côte d’Ivoire). Quantitative determination of total phenolic content using the colorimetric method showed that the EAq contained 228.3 ± 0.67 mg GAE/g of extract, while the EEth 70% contained 226 ± 13.69 mg GAE/g of extract. As for flavonoids, the amount determined for the EAq was 48 ± 8.50 mg QE/g of extract, while the 70% EEth yielded 87 ± 2.40 mg QE/g. The results indicate that the EAq of Lecaniodiscus cupanioides contains more polyphenolic compounds than the 70% EEth. The antioxidant activity of the various extracts was assessed using the DPPH free radical scavenging assay. The antioxidant capacity of the EAq yielded an IC50 of 01.45 ± 0.15 μg/mL, similar to that of vitamin C (IC50= 01.30 ± 0.02 μg/mL) and higher than that of the 70% EEth, which had an IC50 of 02.40 ± 0.60 μg/mL. This antioxidant activity could therefore represent an additional benefit for the use of Lecaniodiscus cupanioides in the treatment of certain conditions related to oxidative stress.

Keywords: Lecaniodiscus cupanioides, antioxidant, polyphenols, Cote d’Ivoire.