Abstract

ZINC OXIDE NANOPARTICLES USING PSEUDOMONAS SP. BROTH CULTURE (PS-ZNO NPS) AND EVALUATION OF THEIR OIL DEGRADATION AND ANTIBACTERIAL ACTIVITY AGAINST PATHOGENS

Ishani Chanda, Nivedita Bhore, Lisa Bhowmick, Priya Shaw, Madhurima Daskarmakar, Pabitra Pal, Ayan Kar, Bishal Dalal, Suman Maity, Dr. Somnath De*

ABSTRACT

The present study investigated the dual application of Pseudomonas sp. in wastewater bioremediation and the antibacterial potential of biologically synthesized Zinc Oxide Nanoparticles (Ps-ZnO NPs). In wastewater treatment systems, Pseudomonas sp. demonstrated effective degradation of organic pollutants under aerobic conditions with continuous aeration. Significant improvements in water quality, including reduced turbidity, decreased foul odor, and enhanced overall clarity, were observed within a few days of incubation. Furthermore, the antibacterial activity of Ps-ZnO NPs was evaluated against selected pathogenic bacteria, namely Salmonella typhi, Escherichia coli, Klebsiella pneumoniae, and Bacillus subtilis, and compared with standard antibiotics. The nanoparticles exhibited varying degrees of antibacterial activity across different bacterial strains. While conventional antibiotics showed superior inhibitory effects against S. typhi and E. coli, Ps-ZnO NPs demonstrated remarkable efficacy against B.subtilis, surpassing the activity of the tested antibiotics. The antibacterial action of ZnO nanoparticles is likely associated with the generation of reactive oxygen species (ROS), leading to oxidative damage of bacterial cellular components. These findings highlight the promising role of Ps-ZnO NPs as an eco-friendly antimicrobial agent and emphasize the bioremediation potential of Pseudomonas sp. in wastewater treatment. However, further studies focusing on mechanism elucidation, synergistic interactions with antibiotics, large-scale production, stability, and in vivo applications are required to fully exploit their therapeutic and environmental potential.

Keywords: Pseudomonas sp., Zinc Oxide Nanoparticles (ZnO NPs), Ps-ZnO NPs, Antibacterial Activity, Bioremediation.