Abstract

THIDIAZURON PROMOTES IN VITRO PLANT REGENERATION AND PHYTOCHEMICAL SCREENING OF GUIZOTIA ABYSSINICA CASS. - A MULTIPURPOSE OIL CROP

Sapna Baghel and Yogendra Kumar Bansal*

ABSTRACT

Objective: Guizotia abyssinica Cass. belonging to family Asteraceae is an herbaceous crop with a lot of industrial as well as medicinal importance. The present study describes a simple, efficient and reproducible regeneration protocol for in vitro propagation of G. abyssinica as well as comparative phytochemical analysis of natural seed, leaf (nature grown as well as in vitro regenerated) and callus of G. abyssinica. Methods: Different explants viz. apical and axilllary buds, leaf and internode were selected for in vitro regeneration study to observe the effect of different concentrations of TDZ. Different parts of the plant like seed, natural leaf, in vitro leaf and callus were dried and extracted in different solvents and were subjected to various phytochemical analysis. Results: Among all the four explants used, apical bud proved best in terms of shoot regeneration and multiplication. The best multiple shoot regeneration (4.44±0.1) was observed on TDZ (0.45 μM) supplemented medium. In vitro regenerated callus showed the presence of phenol. It may be concluded that further standardization of hormonal combinations could be helpful for large scale propagation and extraction of drugs for pharmaceutical application on a commercial scale. Conclusion: The results obtained provide a support for the potential utility of tissue culture strategies for production of bioactive compounds but further studies are required as well.

Keywords: TDZ, Phytochemicals, Guizotia abyssinica, Callus, in vitro regeneration.