Abstract

EFFECT OF COLEUS FORSKOHLII EXTRACT ON THE INDUCTION OF HEPATIC CYTOCHROME P450 ISOENZYME CYP 1A IN RATS

Reethi Budanuru, Sudeep Heggar Venkataramana*, Lingaraju Harakanahalli Basavegowda, Shyam Prasad Kodimule

ABSTRACT

Drug metabolism is one of the major determinants of drug clearance ,if a cytochrome P450 enzyme is induced by a compound, it may either, increase the metabolism of itself (auto induction), or a concurrent therapy, and so reduce plasma levels, resulting in a potential loss of efficacy. Thus the current study was focused to investigate the induction of CYP1A1 in rat liver by Coleus forskohlii root extract (CFE). S9 fraction obtained from Male Wistar rats treated with CFE and β-naphtoflavone (BNF) were used to study the effect on CYP1A1 linked 7-Ethoxyresorufin O-dealkylase (EROD) activity and CYP1A1 mediated mutagenicity of EtBr through AMES assay. EROD activity of S9 obtained from BNF treated was 24.94 folds greater than control. Further studies on mutagenic potential of EtBr in Salmonella typhimurium TA 98 and TA100 showed 24.94 and 25.32 folds increase over control whereas S9 fraction obtained from CFE treated animals increased neither EROD nor the mutagenicity of EtBr. Hence on the basis of Ames and EROD assay our study concludes that ethanolic extract of C. forskohlii prepared at Vidya herbs Pvt. Ltd did not induce CYP1A1 and so also the genotoxicity.

Keywords: Genotoxicity, Cytochrome P450, Coleus forskolii, Ames test.