Abstract

PREBIOTIC EFFECTS OF CASSIA AURICULATA EXTRACTS ON GROWTH, VIABILITY, AND METABOLITE PRODUCTION IN LACTOBACILLUS ACIDOPHILUS: AN IN VITRO STUDY

*K. Anisha Shree, B. Rangeela, J. Ishwarya

ABSTRACT

This study investigates the prebiotic potential of Cassia auriculata and its effect on the growth and functional activity of Lactobacillus acidophilus. Probiotics such as Lactobacillus acidophilus play a crucial role in maintaining gut microbiota balance, enhancing immunity, and preventing gastrointestinal disorders. However, their survival and efficacy depend on suitable substrates, particularly prebiotics. Cassia auriculata, a medicinal plant rich in polyphenols, flavonoids, and dietary fibers, was evaluated for its ability to support probiotic growth. The plant extract was prepared and co-incubated with probiotic cultures under controlled conditions. Growth parameters were analyzed using optical density measurements, Relative Growth Index (RGI), and Prebiotic Index (PI). Results indicated a significant increase in probiotic growth in the presence of Cassia auriculata extract compared to control. The findings suggest a strong synbiotic interaction between the plant extract and probiotic bacteria. This study highlights the potential application of Cassia auriculata as a natural prebiotic ingredient in functional foods and nutraceuticals.

Keywords: Probiotics, Prebiotics, Cassia auriculata, Lactobacillus acidophilus, Synbiotics, Gut Health.