COMPARATIVE STUDY OF CASSYTHA FILIFORMIS AND CLEISTOPHOLIS PATENS FOR ANTIMICROBIAL ACTIVITY
1Adonu Cyril C*,1Eze Chinelo C, 1Ugwueze Mercy E and 2Ugwu Kenneth O
1Department of Pharmaceutics, University of Nigeria Nsukka, Enugu state, Nigeria.
2Department of Microbiology, University of Nigeria Nsukka, Enugu state, Nigeria.
ABSTRACT
The antimicrobial activities of leaf extracts of Cleistopholis patens and
aerial parts of Cassytha filiformis against clinical pathogens were
evaluated and compared by in vitro method. The plant parts were
collected fresh, shade-dried, pulverized and extracted with hot-water,
methanol and n-hexane. Hot-water extraction was by 24 h maceration
while extraction with methanol and n-hexane was done in a Soxhlet
apparatus.The extracts were concentrated and dried separately under
vacuum. The sensitivity of the extracts was done using standard
methods. The respective mean MIC values of the methanol extract
from Cassytha filiformis Linn against Candida albicans, Staph.
aureus, Escherichia coli and Pseudomonas aeruginosa were 10.06
± 0.053 mg/ml, 15.38 ± 0.43 mg/ml, 14.42 ± 0.58 mg/ml and 13.33 ± 1.1 mg/ml while
for hot water extract were 9.39 ± 0.38 mg/ml, 23.13 ± 0.68, 21.35 ± 2.38 mg/ml,
and 10.83 ± 1.32 respectively. Moreso, the mean MIC values of the methanol extract
from Cleistopholis patens against the test isolates were 10.48 ± 0.51 mg/ml (for candida
albicans ), 33.13 ± 1.56 mg/ml (for staph. aureus), 27.69 ± 1.56 mg/ml ( for Escherichia
coli), 17.50 ± 3.31 mg/ml (for Pseudomonas aeruginosa) .The results of statistical analysis
at P< 0.05 showed that the extracts of the Cassytha filiformis Linn are significantly better
antimicrobial agents than the leaf extracts of Cleistopholis patens (Benth) against Candida
albicans,Staph.aureus, E.coli and Ps.aeruginosa.
Keywords: Antimicrobial activity, Cleistopholis patens, Cassytha filiformis, Gentamicin, Nystatin ,Candida albicans, MIC, IZD.
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